LOCUS       40924_44824       14183 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary vector pUB-GWS-Hyg DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14183)
  AUTHORS   Maekawa T, Kusakabe M, Shimoda Y, Sato S, Tabata S, Murooka Y, 
            Hayashi M.
  TITLE     Polyubiquitin Promoter-Based Binary Vectors for Overexpression and 
            Gene Silencing in Lotus japonicus
  JOURNAL   Mol. Plant Microbe Interact. 21 (4), 375-382 (2008)
  PUBMED    18321183
REFERENCE   2  (bases 1 to 14183)
  AUTHORS   Maekawa T, Hayashi M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-MAY-2007) Contact:Makoto Hayashi National Institute of
            Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 
            Kannondai, Tsukuba, Ibaraki 305-8602, Japan
REFERENCE   3  (bases 1 to 14183)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14183)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Plant 
            Microbe Interact."; date: "2008"; volume: "21"; issue: "4"; pages: 
            "375-382"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-MAY-2007) Contact:Makoto Hayashi National Institute of 
            Agrobiological Sciences, Environmental Stress Research Unit; 2-1-2 
            Kannondai, Tsukuba, Ibaraki 305-8602, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(103..277)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(320..1342)
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     promoter        complement(1410..2087)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    2278..2299
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2314..2344
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2352..2368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2376..2392
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(2414..2666)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     protein_bind    2743..2867
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        2892..2922
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2976..3632
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             3977..4279
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(4323..4447)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4481..4735
                     /note="contains intron 1 of Arabidopsis thaliana WRKY 
                     transcription factor 33"
     protein_bind    4790..4914
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(4958..5260)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     CDS             complement(5605..6261)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(6315..6345)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(6370..6494)
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6522..6538)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     gene            complement(6539..7125)
                     /gene="Ljubq1"
                     /label=Ljubq1
     intron          complement(6539..7078)
                     /gene="Ljubq1"
                     /note="5' UTR intron of Ljubq1 gene"
     exon            complement(7079..7125)
                     /gene="Ljubq1"
                     /number=1
     regulatory      complement(7126..7660)
                     /label=Ljubq1 promoter
                     /note="Ljubq1 promoter"
                     /regulatory_class="promoter"
     primer_bind     complement(7687..7703)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    7906..7930
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9230..9856
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             10293..11357
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      11426..11620
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    11964..12104
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(12290..12878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12968..13759)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg atcgacagat
      301 ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt cggtttccac
      361 tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg cgggcgattt
      421 gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga ccctgcgccc
      481 aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca agaccaatgc
      541 ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc cgctcgaagt
      601 agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg ttggcgacct
      661 cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt atgcggccat
      721 tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg acttcggggc
      781 agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca ctgacggtgt
      841 cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat atgaaatcac
      901 gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg ctcgtctggc
      961 taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga acagcgggca
     1021 gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag atgcaatagg
     1081 tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc gcggccgatg
     1141 caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta tttacccgca
     1201 ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg ccctccgaga
     1261 gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg acagacgtcg
     1321 cggtgagttc aggctttttc atatctcatt gccccccggg atctgcgaaa gctcgagaga
     1381 gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg aaatgaactt
     1441 ccttatatag aggaaggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
     1501 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     1561 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
     1621 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
     1681 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
     1741 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
     1801 tggagtagac gagagtgtcg tgctccacca tgttatcaca tcaatccact tgctttgaag
     1861 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
     1921 gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt
     1981 tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat
     2041 ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt
     2101 cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt
     2161 tggcaagctg ctctagccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     2221 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2281 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2341 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2401 cgaattcgag ctcgatctag taacatagat gacaccgcgc gcgataattt atcctagttt
     2461 gcgcgctata ttttgttttc tatcgcgtat taaatgtata attgcgggac tctaatcata
     2521 aaaacccatc tcataaataa cgtcatgcat tacatgttaa ttattacatg cttaacgtaa
     2581 ttcaacagaa attatatgat aatcatcgca agaccggcaa caggattcaa tcttaagaaa
     2641 ctttattgcc aaatgtttga acgatcgatg gcgcgcctag gtaccggatc cctgggtacc
     2701 tctcccgggg atcctctagt ctagaatcga tcggaagtta tcacaagttt gtacaaaaaa
     2761 gctgaacgag aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa
     2821 aaacagacta cataatactg taaaacacaa catatccagt cactatggcg gccgcattag
     2881 gcaccccagg ctttacactt tatgcttccg gctcgtataa tgtgtggatt ttgagttagg
     2941 atccgtcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc actggatata
     3001 ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt cagtcagttg
     3061 ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta aagaccgtaa
     3121 agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc ctgatgaatg
     3181 ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg gatagtgttc
     3241 acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat
     3301 accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg tgttacggtg
     3361 aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc tcagccaatc
     3421 cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac ttcttcgccc
     3481 ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga
     3541 ttcaggttca tcatgccgtc tgtgatggct tccatgtcgg cagaatgctt aatgaattac
     3601 aacagtactg cgatgagtgg cagggcgggg cgtaaacgcg tggatccggc ttactaaaag
     3661 ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata tatactgata
     3721 tgtatacccg aagtatgtca aaaagaggtg tgctatgaag cagcgtatta cagtgacagt
     3781 tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc cggtctggta
     3841 agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat
     3901 caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt tgctgacgag
     3961 aacagggact ggtgaaatgc agtttaaggt ttacacctat aaaagagaga gccgttatcg
     4021 tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga tggtgatccc
     4081 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
     4141 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
     4201 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
     4261 cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg
     4321 accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa
     4381 tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct ttcttgtaca
     4441 aagtggtgat aacccagtcc cgggcgatga attcctgtgg ttggagaagc tagaacctgc
     4501 aaaacaatcc caagaattgt tcagacttca agaaaagaga caaatagcaa taacataatt
     4561 aagaaagagt cgagagttga ctctaaaagt ctttctctct ctttgaaacc gttgacccat
     4621 aactttatca cttccgatca cgattcattg tcaagatgtt aaatcaatga tacatcaaac
     4681 acagattatc atcactaatt ggattcattt caaaagaaac agagaaaaaa aacagagagg
     4741 cttacgttag cagaggagga gaagcttcta gcccatggaa cggaggatca ccactttgta
     4801 caagaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     4861 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggtcgac
     4921 ctgcagactg gctgtgtata agggagcctg acatttatat tccccagaac atcaggttaa
     4981 tggcgttttt gatgtcattt tcgcggtggc tgagatcagc cacttcttcc ccgataacgg
     5041 agaccggcac actggccata tcggtggtca tcatgcgcca gctttcatcc ccgatatgca
     5101 ccaccgggta aagttcacgg gagactttat ctgacagcag acgtgcactg gccaggggga
     5161 tcaccatccg tcgcccgggc gtgtcaataa tatcactctg tacatccaca aacagacgat
     5221 aacggctctc tcttttatag gtgtaaacct taaactgcat ttcaccagtc cctgttctcg
     5281 tcagcaaaag agccgttcat ttcaataaac cgggcgacct cagccatccc ttcctgattt
     5341 tccgctttcc agcgttcggc acgcagacga cgggcttcat tctgcatggt tgtgcttacc
     5401 agaccggaga tattgacatc atatatgcct tgagcaactg atagctgtcg ctgtcaactg
     5461 tcactgtaat acgctgcttc atagcacacc tctttttgac atacttcggg tatacatatc
     5521 agtatatatt cttataccgc aaaaatcagc gcgcaaatac gcatactgtt atctggcttt
     5581 tagtaagccg gatccacgcg tttacgcccc gccctgccac tcatcgcagt actgttgtaa
     5641 ttcattaagc attctgccga catggaagcc atcacagacg gcatgatgaa cctgaatcgc
     5701 cagcggcatc agcaccttgt cgccttgcgt ataatatttg cccatggtga aaacgggggc
     5761 gaagaagttg tccatattgg ccacgtttaa atcaaaactg gtgaaactca cccagggatt
     5821 ggctgagacg aaaaacatat tctcaataaa ccctttaggg aaataggcca ggttttcacc
     5881 gtaacacgcc acatcttgcg aatatatgtg tagaaactgc cggaaatcgt cgtggtattc
     5941 actccagagc gatgaaaacg tttcagtttg ctcatggaaa acggtgtaac aagggtgaac
     6001 actatcccat atcaccagct caccgtcttt cattgccata cggaattccg gatgagcatt
     6061 catcaggcgg gcaagaatgt gaataaaggc cggataaaac ttgtgcttat ttttctttac
     6121 ggtctttaaa aaggccgtaa tatccagctg aacggtctgg ttataggtac attgagcaac
     6181 tgactgaaat gcctcaaaat gttctttacg atgccattgg gatatatcaa cggtggtata
     6241 tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg acggatccta
     6301 actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6361 tgcggccgcc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     6421 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttt
     6481 ttgtacaaac ttgtgatcct caggtggatc ctctttctcg agatccacta gttctagact
     6541 gtaatcacat caacaacaga taaatcagaa tccaacacac gaaattgaaa caagtgaaaa
     6601 ccctaattaa cgcttggatc aaacagatta agagtggatc taacatcaac agataaatca
     6661 gaatccataa caagaaattg aaacaagtga aaaccctaat tgaagcttgg atcaaacaga
     6721 ttaagagtgg atctaacaat aatttccaat ttgaataaat tatttgacat aattccataa
     6781 cggatctgaa gaattcaatt ggaaacccta attaatctgc aattgctaaa ccgaaatcga
     6841 ttcctaacaa aactgaacca caattatcat agatctacat cgcaagccat cgaaaacatt
     6901 gataaaacaa gatctgagat tcaagctgta aaattcaatt tcaaaccgat ctcaattttg
     6961 gaaaatcaca aaaaattcct agatctcatg cttcgaaaca agaaaactac ctatgattga
     7021 tcggaaacaa aactcaacga aaggattaca gagaaagaag agagaaattg gatcatacct
     7081 tgaaagtaga gagaagcgat atgaacaaga attgttaggg ttgggaactg aattgtgaag
     7141 aagctaaggg gattggaggt aatttatagc aaaagcggtg aaattattaa ccagtcaaat
     7201 tgtgacacgt gtccttatga cgcgtcacta gttgcgtgtg gtttgggata ggatacgttg
     7261 tgtggttgag ttcttcttgg aaacggtagt tgcaaattgc aagttgttgg cggtttgtta
     7321 atttcacgga gttaaacgca tctcgccgcc ttttgagttt ggattgataa atcgttacaa
     7381 cggcgttact ttgctgggcc ccgttttttt tgaattgggc caccgcacca gtgcaagttg
     7441 ggggttcctt ggttttccag aatgggctcc actctctatg attgattgga ggaggccggc
     7501 tgctcagaaa ttttataata agttgttata tacttctccc cttaaaataa gtgatttttg
     7561 tctatccata gttgcaataa gttgtactaa caacttatct aaattacttt atcaataata
     7621 caagtaattc aattaattat ttcctcaaaa tcctctctcc atcgaattcc tgcagcccag
     7681 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     7741 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     7801 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgctagagca gcttgagctt
     7861 ggatcagatt gtcgtttccc gccttcagtt taaactatca gtgtttgaca ggatatattg
     7921 gcgggtaaac ctaagagaaa agagcgttta ttagaataac ggatatttaa aagggcgtga
     7981 aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat
     8041 caaagtactt tgatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg
     8101 cagccgtctt ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc
     8161 ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc
     8221 gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat
     8281 gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc
     8341 ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg
     8401 gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc
     8461 ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg
     8521 ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg
     8581 ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg
     8641 agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc
     8701 ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag
     8761 gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag
     8821 gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag
     8881 gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc
     8941 aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg
     9001 ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg
     9061 ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg
     9121 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg
     9181 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat
     9241 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg
     9301 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc
     9361 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc
     9421 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc
     9481 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc
     9541 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca
     9601 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa
     9661 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct
     9721 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt
     9781 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat
     9841 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg
     9901 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca
     9961 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg
    10021 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg
    10081 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg
    10141 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg
    10201 aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac
    10261 tggaaccccc aagcccgagg aatcggcgtg acggtcgcaa accatccggc ccggtacaaa
    10321 tcggcgcggc gctgggtgat gacctggtgg agaagttgaa ggccgcgcag gccgcccagc
    10381 ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg gcaagcggcc gctgatcgaa
    10441 tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc gtcgattagg aagccgccca
    10501 agggcgacga gcaaccagat tttttcgttc cgatgctcta tgacgtgggc acccgcgata
    10561 gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa gcgtgaccga cgagctggcg
    10621 aggtgatccg ctacgagctt ccagacgggc acgtagaggt ttccgcaggg ccggccggca
    10681 tggccagtgt gtgggattac gacctggtac tgatggcggt ttcccatcta accgaatcca
    10741 tgaaccgata ccgggaaggg aagggagaca agcccggccg cgtgttccgt ccacacgttg
    10801 cggacgtact caagttctgc cggcgagccg atggcggaaa gcagaaagac gacctggtag
    10861 aaacctgcat tcggttaaac accacgcacg ttgccatgca gcgtacgaag aaggccaaga
    10921 acggccgcct ggtgacggta tccgagggtg aagccttgat tagccgctac aagatcgtaa
    10981 agagcgaaac cgggcggccg gagtacatcg agatcgagct agctgattgg atgtaccgcg
    11041 agatcacaga aggcaagaac ccggacgtgc tgacggttca ccccgattac tttttgatcg
    11101 atcccggcat cggccgtttt ctctaccgcc tggcacgccg cgccgcaggc aaggcagaag
    11161 ccagatggtt gttcaagacg atctacgaac gcagtggcag cgccggagag ttcaagaagt
    11221 tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct gccggagtac gatttgaagg
    11281 aggaggcggg gcaggctggc ccgatcctag tcatgcgcta ccgcaacctg atcgagggcg
    11341 aagcatccgc cggttcctaa tgtacggagc agatgctagg gcaaattgcc ctagcagggg
    11401 aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta cattgggaac ccaaagccgt
    11461 acattgggaa ccggaacccg tacattggga acccaaagcc gtacattggg aaccggtcac
    11521 acatgtaagt gactgatata aaagagaaaa aaggcgattt ttccgcctaa aactctttaa
    11581 aacttattaa aactcttaaa acccgcctgg cctgtgcata actgtctggc cagcgcacag
    11641 ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg ctccctacgc cccgccgctt
    11701 cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc tggcctacgg ccaggcaatc
    11761 taccagggcg cggacaagcc gcgccgtcgc cactcgaccg ccggcgccca catcaaggca
    11821 ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
    11881 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    11941 gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
    12001 tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
    12061 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct
    12121 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    12181 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    12241 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    12301 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    12361 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    12421 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    12481 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    12541 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    12601 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    12661 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    12721 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    12781 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    12841 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    12901 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcattcta
    12961 ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga
    13021 tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg
    13081 accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa
    13141 taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg
    13201 aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat
    13261 ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca
    13321 gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata
    13381 tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc
    13441 tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat
    13501 tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca
    13561 gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt
    13621 ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag
    13681 acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg
    13741 atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc
    13801 ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct
    13861 taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc
    13921 gacggagccg attttgaaac cgcggtgatc acaggcagca acgctctgtc atcgttacaa
    13981 tcaacatgct accctccgcg agatcatccg tgtttcaaac ccggcagctt agttgccgtt
    14041 cttccgaata gcatcggtaa catgagcaaa gtctgccgcc ttacaacggc tctcccgctg
    14101 acgccgtccc ggactgatgg gctgcctgta tcgagtggtg attttgtgcc gagctgccgg
    14161 tcggggagct gttggctggc tgg
//
